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Life Science Equipment. Label-free Live Cell Imaging
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Nanolive 3D Cell Explorers
A stem cell differentiation story
In this video, we have created a timeline showcasing the temporal responses of human mesenchymal stem cells to neurogenic differentiation media. All images were taken using Nanolive’s CX-A (nanolive.ch/products/3d-microscopes/cx-a/):
In the first video, refractive index images were captured once every 7 mins for 12 h, using the 3×3 gridscan mode (a field of view of 275×275 µm).
Stem cell differentiating into neuronal cells
In this video, we observe the morphological changes umbilical cord matrix human mesenchymal stem cells undergo upon exposure to neurogenic differentiation media
Images were captured once every 4.5 mins for 22 h using the 5×5 gridscan mode (a field of view of 450×450 μm) on Nanolive’s CX-A (nanolive.ch/products/3d-microscopes/fluo/).
Label-free Live Cell Imaging: Diving into the immune system - APC T-Cells interaction/activation experiment
In this video we see the T-Cell activation totally marker-free. Pre-stimulated antigen presenting cells (APCs*: dendritic cells and macrophages) are cultured with freshly isolated “naïve T-cells”, the OT-I mice T-cells in order to teach them to recognize and kill the tumor cells.
* APCs are special cells that present the antigens on their surface so that other immune cells can “see” and detect it.
Technology:
3D Cell Explorer microscope from Nanolive, Switzerland.
Labelfree microscopy: Long Term Live Imaging of Mouse Pre-adipocytes (48hrs)
Live imaging of mouse pre-adipocytes for 48 hours. 1 image per minute. This is label-free yet you can see amazing biology unfolding under your eyes! This is unique material as never seen before.
Technology: 3D Cell Explorer microscope from Nanolive, Switzerland.
Multi-well, time-lapse imaging of live pre-adipocytes perturbed by different drugs | Nanolive imaging
In this video, we observe the response of pre-adipocyte cells grown in 96 well plates to Tamoxifen, Raptinal, and Shikonin. Drugs were applied at various concentrations, with or without the fluorescent probe MitoTracker added. Unperturbed cells were included as a negative control.
3D CELL EXPLORER
3D CELL EXPLORER-FLUO
